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| Karyn M. Usher |
| Assistant Professor |
| Department of Chemistry |
| West Chester University of Pennsylvania |
| West Chester, PA 19383 |
| Office Location: Schmucker Science Center 331 |
| Phone: (610) 436-2622 |
| Fax: (610) 436-2890 |
| email:
kusher@wcupa.edu
|
Karyn M. Usher B.A., Ph.D. [Florida State University] Assistant Professor since August 2005
| Course Code | Room | Course Title | Day | Time |
| CHE 321-01 | SS 107 | Introduction to Analytical Chemistry | Tues/Thurs | 8-9:15am |
| CRL 321-01 | SS 215 | Analytical Chemistry Lab I | Friday | 1-5pm |
| CRL 321-80 | SS 215 | Analytical Chemistry Lab I | Wednesday | 5-9pm |
Office Hours:
Tuesday 10-12 noon
Thursday 10-12 noon
Friday 9-10 am
Research Interests and Student Research opportunities
Chromatography is a separation technique that is used in virtually all branches of science and technology. In the chromatographic process, a mixture is introduced into the system that has been chosen for the separation, and as the mixture passes through the system different types of molecules will move at different migration rates, thus effecting a separation. Chromatography is the most widely used of all separation techniques, and this is because of its versatility and its separation power. It is a very powerful method that can analyze even extremely complex mixtures. There are two main categories of chromatography, liquid chromatography and gas chromatography. Within these two categories there are many different modes that can be utilized, and these differ in many features, such as the size of the columns used, the packing materials, or even the pressures and temperatures at which the separation is performed. It is usually possible to find a chromatographic technique that is well suited to whatever analysis needs to be performed, due to the many different types available. There are many things that can be varied when performing chromatographic experiments. These include changing the type of mobile phase, the mechanism of retention, the incoming solute concentration profile, the column and planar procedures, the gradient methods, dimensionality, or even just the physical scale of the column. Each of these changes can be used to enhance the separation from a poor separation to a good separation.
Although chromatography is widely used and embraced by scientists involved in many different types of research, there are still many questions about some of the fundamental aspects of this technique. Many of these questions have been investigated previously, but there has been no agreement in the literature about the theory behind many of the fundamental processes. Although everyday it becomes possible to perform more and more difficult separations, each time one of these difficult separations is achieved a more difficult one presents itself. Because of this, there is still a need for scientists to perform fundamental research in this field. Perhaps if the mechanisms behind the separations can be better understood, scientists can decide how to go about improving the technique. By doing so, chromatography can become an even more useful technique.
As the solutes move through the columns and are separated, the band of each analyte is broadened which gives us the peak shape that we see in liquid chromatography. This is shown in the diagram below. In 1956, van Deemter described the band broadening in a chromatographic system using the van Deemter equation. The A term represents eddy diffusion, the B term represents molecular diffusion, the C term represents resistance to mass transfer and u is the mobile phase velocity. Other equations have since been introduced to model efficiency data, and these include the equations of Giddings, Huber and Hulsman, Horvath and Lin, and Knox.

H = A + B/u + Cu

Band broadening even occurs outside the column in the connection tubing, detector cells, injector etc. This type of broadening is called extra-column broadening. The graph below shows what a graph of extra column variance (ECV) versus flow rate would be expected too look like. As you can see, the ECV is dependent on the flowrate and its contribution becomes smaller as the flow rate is increased. One project in my lab investigates the effects of ECV on different separations. When the ECV is subtracted we get what is called the column variance and this includes all the band broadening that is occurring due to processes inside the column. The second graph below shows the same data both with and without the extra column variance subtracted. The points represent the total variance (column and extra column variance) and the lower curve represents the column variance (extra column variance subtracted). The k’ for benzene at these mobile phase strengths is as follows: 70% ACN, k’=1.4; 80% ACN, k’=0.9; and 90% ACN, k’=0.5

There are other projects being performed in the lab, email me for more information.

Back: Karyn Usher (advisor), Anna Glinko (S'08), Michael Bozym (F'07, S'08)
Front: Michelle Owens, (F'07, S'08), Casey Mulcahy (F'06, S'07, F'07, S'08)
Previous Research Students:
Andrew Newsome (F'05, S'06), Margaret Dudley(F'05,S'06), Scott Snyder(S'06, F'06, S'07, F'07), Sarat Gnanamgari (S'06), Andria Styborski (F'06, S'07), Dina Konetski (F'06),
Amber Gegg (S'07)
Chromatography Forum of the Delaware Valley Student Award M. Bozym/A. Glinko/M. Owens Spring 2008
Chromatography Forum of the Delaware Valley Student Award C. Mulcahy/S. Snyder Spring 2008
CAS Student Research Award Casey Mulcahy Fall 2007
Eastern Analytical Symposium Student Research Award Andria Styborski Fall 2007
Sartomer Environmental Award Casey Mulcahy Fall 2007-Spring 2008
CAS Student Research Award A. Styborski/S. Snyder Spring 2006
Sartomer Environmental Award Andria Styborski Fall 2006-Spring 2007
Sartomer Environmental Award Andrew Newsome Fall 2005-Spring 2006
Grants:
Multicultural Faculty Commission 2008 Grant Spring 2008
Agilent Technologies Chromatography Grant Summer 2007
CAS Entrepreneur grant Spring 2006
CAS Faculty Development Award Fall 2005
Sponsored Research Incentive Award Fall 2005
Usher, Karyn M., Styborski, Andria L, Snyder, Scott H. “Extra Column Effects in HPLC: Peak Width Versus Symmetry” Eastern Analytical Symposium, Somerset, NJ, Nov 12, 2007
Usher, Karyn M, Styborski, Andria L, Snyder, Scott H. “Dispersion: Following a Band of Molecules Through the Chromatographic System” Invited seminar as part of the annual seminar program, American University of Beirut, Beirut, Lebanon, June 27, 2007
Styborski, Andria L., Snyder, Scott H., Usher, Karyn M. “Systematic Investigation of the Magnitude of Extra Column Dispersion in High Performance Liquid Chromatography” 39th Mid-Atlantic Regional Meeting of The American Chemical Society. May 17, 2007
Snyder, Scott H., Styborski, Andria L., Usher, Karyn M. “The Extra Column Effect: Peak Width vs. Symmetry” West Chester University All Sciences Poster Session. May 3, 2007
Styborski, Andria L., Snyder, Scott H., Usher, Karyn M. “The Effects of Extra Column Volume on Chromatographic Separations in HPLC Systems” West Chester University annual Research Day. March 29, 2007
Florida State University - Chemistry
Chromatography Forum of the Delaware Valley
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